Inhibitory effect of pterin acid against ricin and recombinant ricin A chain
- VernacularTitle:蝶酸对天然蓖麻毒素及重组蓖麻毒素A链蛋白的拮抗作用研究
- Author:
Xi-yuan, CAO
;
Qing, ZHAO
;
Yan, LI
;
Bei-fen, SHEN
;
Yu-xia, WANG
;
Jian-nan, FENG
;
Hui, PENG
- Publication Type:Journal Article
- Keywords:
pterin acid;
ricin;
recombinant ricin A chain protein;
cell-free system;
inhibitor
- From:Bulletin of The Academy of Military Medical Sciences
2010;34(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effect of pterin acid (PTA) against ricin and recombinant ricin A chain protein. Methods Luciferase protein synthesis inhibition assay in a cell-free system and in vitro cytotoxicity experiments were performed to assess the biological activity of ricin and rRTA treated with PTA.Results The result showed that PTA could significantly inhibit the activity of ricin and rRTA in a dose-dependent manner.Conclusion PTA might be used as a small molecular probe to develop an evaluating system for ricin/RTA small molecular inhibitor in vitro. The cell-free system adopted in the current study could also serve as a necessary basis for screening some novel small molecular compounds against ricin and RTA in the future.
