Cloning and sequencing of partial gene from SENV isolate in China
- VernacularTitle:SEN病毒部分基因的克隆及序列测定
- Author:
LinSheng ZHAN
;
Qinghua, MENG
;
ShuaiZheng JIA
;
Hongyan, SUN
;
ZhiYan, DU
;
Quanli, WANG
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(2):88-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the presence of SENV infection among patients in China,and analyze partial nucleotide sequence of SENV isolated from a patient with non A-G hepatitis.Methods:A nested polymerase chain reaction (PCR) assay with primers from ORF1 of SENV genome was established to detect SENV DNA.The PCR product was cloned and sequenced.Results:SENV DNA was positive in 2 of 7 patients with non A-G hepatitis and TTV negative.Partial gene of a SENV isolate was compared with the corresponding region of SENV isolate(AX025730)from Italy and was found that the nucleotide homology was 90%.Conclusions:The results of this study confirmed the presence of SENV infection in China.The development of a PCR assay for SENV DNA detection and the cloning,sequencing of the SENV isolate have important implication for the diagnosis and epidemiological investigation on SENV infection.
