Experimental study of 99Tcm-tri-peptide as a novel tumor imaging agent
10.3760/cma.j.issn.0253-9780.2011.02.012
- VernacularTitle:99Tcm标记亲肿瘤三肽的实验研究
- Author:
Wen-hui, XIE
;
Xiao-jia, CAI
;
Ci-yi, LIU
;
Jun, ZENG
;
Li-hua, ZHANG
;
Bei, LEI
;
Gang, HUANG
- Publication Type:Journal Article
- Keywords:
Peptides;
Technetium;
Isotope labeling;
Neoplasms;
Inflammation;
Rabbits;
Mice,nude
- From:Chinese Journal of Nuclear Medicine
2011;31(2):121-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate 99Tcm-Arg-Glu-Ser (99Tcm-RES) as a potential tumor imaging agent. Methods RES was synthesized using solid phase peptide synthesis. The optimal labeling conditions of RES were determined under different reagents and reacting temperatures using SnC12 as reducing agent.The biodistribution of 99Tcm-RES was studied in nude mice bearing human lung cancer A549. Results The radiochemical purity of 99Tcm-RES was up to 85% and the radiochemical purity was 75% ever after 6 h at room temperature. The tumor uptake of 99Tcm-RES was obvious and the radioactivity ratios of tumor/blood,tumor/heart, tumor/liver, tumor/lung, tumor/spleen and tumor/muscle were 5.31, 1.88, 1.57, 3.58,4. 16 and 5.92, respectively at 6 h after 99Tcm-RES injection. Gamma camera imaging showed that tumor uptake of 99Tcm-RES was negative in rabbits with inflammatory mass but positive in those bearing tumor.The radioactivity ratio of tumor/inflammation was 3.12 at 6 h after injection. Conclusion 99Tcm-RES might possibly become a potential tumor imaging agent.
