Synthesis and biodistribution of a bi-functional agent 99Tcm-Gd-DTPA-DG in tumor bearing nude mice
10.3760/cma.j.issn.0253-9780.2011.02.011
- VernacularTitle:双功能制剂99Tcm-Gd-DTPA-DG的制备及在荷瘤裸鼠体内的生物分布
- Author:
Wei, ZHANG
;
Liang, CAI
;
Yue, CHEN
;
Zhan-wen, HUANG
;
Zhi-ling, DING
;
Feng, CAO
;
Li, ZHANG
;
Ling, HE
- Publication Type:Journal Article
- Keywords:
Deoxyglucose;
Gadolinium;
DTPA;
Technetium;
Neoplasm transplantation;
Mice,nude;
Radionuclide imaging
- From:Chinese Journal of Nuclear Medicine
2011;31(2):117-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the stability and biodistribution of a novel SPECT-MRI bi-functional agem 99Tcm-Gd-DTPA-DG in tumor-bearing nude mice. Methods DTPA-DG was synthesized and then conjugated with Gd2O3 to generate Gd-DTPA-DG. The tumor-bearing nude mice were scanned by MRI to evaluate the tumor targeting ability of Gd-DTPA-DG. The orthogonal experiment was applied to optimize pH value of reaction medium and reaction temperature. The radiolabeling efficiency was measured by thin layer chromatography. The distribution of 99Tcm-Gd-DTPA-DG in nude mice was evaluated by scintigrapy in vivo. The % ID/g was measured at different time after intravenous injection of 99Tcm-Gd-DTPA-DG. Results The tumor was significantly enhanced by Gd-DTPA-DG with MRI. The radiochemical purity of 99Tcm-Gd-DTPADG was about 98.5% and remained 96.2% at room temperature for 6 h. The tumor was well visualized by 99TcmGd-DTPA-DG SPECT at 2 h after injection. The tumor uptake was (1.48 ±0.12) %ID/g, and the rumor to muscle radioactivity ratio was 2.91. Conclusions MRI contrast of Gd-DTPA-DG may enhance tumor detection. 99Tcm-labeled Gd-DTPA-DG may be useful for tumor imaging and might have a potential role as a SPECT-MRI bi-functional agent.
