Detection of plague IgM antibody in the serum of herding dogs by capture enzyme-linked immunosorbent assay
10.3760/cma.j.issn.1000-4955.2012.06.024
- VernacularTitle:酶联免疫吸附试验捕获法检测牧犬血清中鼠疫IgM抗体
- Author:
Xin-hui, WANG
;
En-jie, XU
;
Gang, LEI
;
Li-fu, LIAO
;
Turd, RENA
;
Jie, TU
;
Mattuhut ABULYMIT
;
Bing, LI
;
Bing-chen, XU
- Publication Type:Journal Article
- Keywords:
Plague;
Immunoglobulin M;
Enzyme-linked immunosorbent assay
- From:Chinese Journal of Endemiology
2012;31(6):681-683
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a capture enzyme-linked immunosorbent assay (ELISA) for detection of plague IgM antibody in herding dogs.Methods ELISA plates were coated with serum IgM antibody against dogs and F1 antibody to plague in the serum of herding dogs was detected by a sandwich ELISA.Results A total of 216 serum samples of herding dogs were tested,26 were positive for plague F1 antibody and the positive rate was 12.03％ (26/216); 14 were positive for plague IgM antibody and the positive rate was 6.48％ (14/216); IgM positive accounted for 53.8％(14/26) of all positive samples.Conclusions Serum plague IgM antibody of herding dogs can be used to predict the prevalent time and distribution of recent animal plague in plague foci indirectly,and to provide reference information for timely implementation of control measures.
