Effects of chronic fluorosis on 4.8 kb mitochondrial DNA in liver, kidney and brain of rats
10.3760/cma.j.issn.2095-4255.2013.02.002
- VernacularTitle:慢性氟中毒对大鼠肝、肾和脑皮质细胞线粒体DNA 4.8 kb的影响
- Author:
Di-dong, LOU
;
Kai-lin, ZHANG
;
Shuang-li, QIN
;
Yan-fei, LIU
;
Yan-jie, LIU
;
Zhi-zhong, GUAN
- Publication Type:Journal Article
- Keywords:
Fluorosis;
Rats;
Mitochodrial DNA
- From:Chinese Journal of Endemiology
2013;(2):121-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the deletion pattern of 4.8 kb mitochondrial DNA(mito-DNA) in liver,kidney,and brain of rats with chronic fluorosis and to explore the significance of mitochondria in the pathogenesis of chronic fluorosis.Methods Sixty SD rats were randomly divided into 3 groups according to body mass (20 in each group):control,low-fluoride and high-fluoride groups,and they were fed with different concentrations of fluoride in drinking water (0,10,50 mg/L,respectively) for 6 months.Mito-DNA in liver,kidney and brain was detected by real-time PCR.Results The amounts of 4.8 kb mito-DNA in liver(2.1 × 10-3,1.6 × 10-3),kidney (1.7 × 10-3,1.4 × 10-4) and brain cortex (1.5 × 10-5,1.3 × 10-5) in low-and high-fluoride groups were significantly reduced,as compared with that of control group (2.9 × 10-3,2.0 × 10-3,1.1 × 10-4,all P ＜ 0.05).The amount of 4.8 kb mito-DNA in kidney in high-fluoride group was lower than that in low-fluoride group (P ＜ 0.05).Conclusions Excessive fluoride intake can result in missing of 4.8 kb mito-DNA in liver,kidney and brain cortex.The abnormal of mito-DNA might be related to the dysfunction of mitochondrial respiratory chain.
