Comparison of the two-dimensional electrophoresis maps of rat spinal cord protein extracted by two different solution systems
- VernacularTitle:两种不同溶液体系提取大鼠脊髓蛋白双向电泳图谱比较
- Author:
Qin-Xue, DING
;
Yu-feng, JIA
;
Cong-Jian, ZHAO
;
Hai-ping, QUE
;
Shao-Jun, LIU
;
Yao-Jun, GUO
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(1):17-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the two-dimensional electrophoresis(2-DE) maps of rat spinal cord protein extracted by two different solution systems.Methods: Adult rat spinal cord protein was precipitated with 10% trichloracetic acid in acetone and resuspended in 8 mol/L urea plus 4%CHAPS (A solution) or, 5 mol/L urea, 2 mol/L thiourea, 2%CHAPS plus 2%SB3-10 (B solution). One hundred and fifty micrograms of protein was loaded on 18 cm IPG strip holder and run isoelectric focusing electrophoresis as the first dimension, then horizontal SDS-PAGE as the second dimension. Protein spots were visualized by silver stain.Results:There were 1 059 and 1 023 protein spots in each map, of which 790 spots were matched in two maps. There were 269 and 233 spots exclusively extracted by A and B solutions, respectively. Taken together, 1292 different spots were totally obtained by A and B solutions.Conclusion: Integrating protein spots extracted by different solution systems is beneficial for achieving intact 2-DE map of tissues.
