Study of interaction between NS3 serine protease of HCV and wild type P53
- VernacularTitle:HCV NS3丝氨酸蛋白酶与野生型P53相互作用的研究
- Author:
Wu, OU
;
E-De, QIN
;
Cui-hong, YANG
;
Pei-ying, YANG
;
Man, YU
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(1):21-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the molecular interaction between non-structural protein 3 serine protease of hepatitis C virus(HCV)and wild type P53,and to lay the basis for elucidating the mechanism of oncogenesis of hepatocellular carcinoma(HCC)after infection of HCV.Methods:The recombinant plasmids,pGAD424-NS3,pGAD424-NS315aa- and pGAD424-NS330aa-,were constructed and the interaction between NS3 serine protease and its cofactor NS4A,the interaction between wild type P53 and NS3 serine protease and its N-truncated mutants were dectected qualitatively and quantitatively in yeast two-hybrid system.Results:The results indicated that interaction existed not only between full-length NS3 serine protease and P53,but also between N-truncated mutants of NS3 serine protease and P53.Furthermore,the difference between enzyme activity unit(IU)of β-gal induced by these interactions was not significant(P>0.05).Conclusions:NS3 serine protease of hepatitis C virus and its N-truncated mutants can interact with wild type P53,and the region of NS3 serine protease involved in the interaction may be located in its C-terminal,but not in its N-terminal.
