Isolation, purification, primary culture and identification of alveolar type Ⅱ epithelial cell from rats
- VernacularTitle:大鼠肺泡Ⅱ型上皮细胞的分离、纯化、原代培养及鉴定
- Author:
Ge, MENG
;
Jian, ZHAO
;
Xin-huai, L(U)
;
Ri-gao, DING
- Publication Type:Journal Article
- Keywords:
alveolar type Ⅱ epithelial cell;
isolation;
purification;
primary culture;
identification
- From:Bulletin of The Academy of Military Medical Sciences
2010;34(1):34-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of isolation, purification, primary culture and identification of alveolar type Ⅱ epithelial cells(AT-Ⅱ).Methods The AT-Ⅱs were isolated from Wistar rats by trypsin,purified by differential centrifugation, erythrocyte spallation, differential adherence and immune adherence, and identified by observing the morphology of cultured cells under the inverted phase and tannic acid staining. Results and Conclusion The cultured primary AT-Ⅱs in vitro presented single or island form growth, and their shapes were round or elliptical. A great deal of fine particles showed sharp contrast, and were observed in intracytoplasm. The cell nuclei were clear. They were positive for tannic acid staining.The primary culture AT-Ⅱs obtained from improved isolation and purification have good growth state and purity, and are suitable for research in vitro.
