Primary culture of human vascular smooth muscle cells and their calcification mode
- VernacularTitle:人血管平滑肌细胞的原代培养及其钙化模型的建立
- Author:
Xiao-zhong, CHENG
;
Ting, SONG
;
Bei, HUANG
;
Hui, ZHONG
- Publication Type:Journal Article
- Keywords:
human embryo vascular smooth muscle cells;
primary culture;
calcification mode
- From:Bulletin of The Academy of Military Medical Sciences
2010;34(1):37-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a calcification mode in vitro of human vascular smooth muscle cells (HVSMCs) induced by β-GP. Methods Primary HVSMCs were obtained from human embryo by plant method and confirmed by stain with α-sm-actin antibody. The cells after 4-6 passages were divided into two groups.The control group was incubated with normal DMEM medium while the calcification group was incubated with the medium containing 10 mmol/L β-glycerophosphate for 10 days.Calcification was confirmed by alizarin red S stain and alkaline phosphatase(ALP) assays. Results The primary cells observed by S-P stain were positive and the cells after being stained were pale yellow. After being induced with β-GP, the cells of calcification group began concentric growth and formed vesicles. Alizarin red S staining showed that the reaction of calcifying nodules was red,ALP activity was higher than that of controls at various time points(4 d,6 d,8 d and 10 d,P＜0.01 ).Conclusion The HVSMCs could be induced into calcification in vitro by β-GP, and this model contribates to further studies of vascular diseases.
