Preliminary evaluation on the detection of serum level of antibody to Bacillus anthracis by enzyme-linked immunosorbent assay using crude antigen
- VernacularTitle:粗提抗原检测炭疽血清抗体ELISA方法的初步评价
- Author:
Jian-chun, WEI
;
Hui-juan, ZHANG
;
Feng-qin, MA
;
En-min, ZHANG
;
Dong-zheng, YU
- Publication Type:Journal Article
- Keywords:
Bacillus anthracis;
Enzyme-linked immunosorbent assay;
Antibodies
- From:Chinese Journal of Endemiology
2008;27(3):329-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the method of detecting antibodies to Bacillus anthracis by enzymelinked immunosorbent assay(ELISA)using crude antigen.Methods The anti-Bacillus anthracis antibody levels in sera of 42 healthy people and 42 patients were detected by indirect ELISA.Standard curve was plotted using the data from positive controls,based on which the relative content of each serum was calculated and compared with the result of rLF.Results The median of antibody's relative content in patient group and healthy people group are 1.19 and 0.24,the differences being statistically significant(uc=7.643,P＜0.05).The result of crude antigen is in concordance with rLF(but not parallel absolutely).Conclusions Crude antigen can distinguish most of patients with healthy population effectively.The results suggested that crude antigen is applicable in anti-Bacillus anthracis antibody surveillance.
