Application of flowing injection of hydride generation atomic fluorometric method to assay blood selenium content
- VernacularTitle:应用流动注射氢化物发生原子荧光法检测全血硒
- Author:
Cheng-bo, CHE
;
Jun-rui, PEI
;
Shao-chen, LI
;
Ming-fa, LIU
;
Li, SONG
;
Yu, WANG
;
Yang, LIU
;
Ling-wang, ZHOU
;
Tong, WANG
- Publication Type:Journal Article
- Keywords:
Selenium;
Blood;
Flowing injection hydride generation atomic fluorometric method;
2,3-diaminonaphthalene fluorometric method
- From:Chinese Journal of Endemiology
2008;27(5):555-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective Using flowing injection hydride generation atomic fluotometric method(FI-HG- AFM)to detect the whole blood selenium content.Methods A series of standard selenium(1,2,4,6,8.10μg/L) were dectecte by FI-HG-AFM,and the precision and accuracy of the method were observed.The selenium contents of 89 blood samples were detected by FI一HG-AFM and 2,3-diaminonaphthalene fluorometric method(DFM) respectively,and the correlation of two methods Was analyzed.Results The detection limit and recovery rate of FI-HG-AFM were 0.138μg/L and 96.4%,respectively.The relative standard deviation(RSD)was maximized at 1 μg/L(4.406%).The RSD diminished along with the increase of the concentration,and it declined to 0.512%at 10 μg/L.There wag no significant difference(t=1.7878,P>0.05)between the result8 of FI-HG-AFM[(45.9± 14.5)μg/L]and DFM[(47.5 ±13.3)μg/L],and the two methods were in highly positive correlation(r=0.8143,P< 0.01).Conclusions The whole blood selenium content call be rapidly and exactly detected by FI-HG-AFM,and the method are highly consistent with DFM.
