Expression of bone morphogenetic protein-2 and bone morphogeneflc protein-7 in the knee joint synovial tissue of fluorosis rats
10.3760/cma.j.issn.1000-4955.2009.01.008
- VernacularTitle:骨形态发生蛋白-2和骨形态发生蛋白-7在氟中毒大鼠膝关节滑膜中的表达
- Author:
Jia-shun, ZENG
;
Long, LI
;
Ying-man, MO
;
Mao-juan, YU
;
Chun-ling, ZHU
- Publication Type:Journal Article
- Keywords:
Fluoride poisoning;
Coal;
Bone morphogenefic proteins;
Arthritis;
Synovial membrane
- From:Chinese Journal of Endemiology
2009;28(1):28-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expressions of bone morphogenetic protein-2(BMP-2)and bone morphogenetie protein-7(BMP-7)in the synovial tissue of fluorosis rats and its correlation with pathogenic mechanism of fluorosis arthritis.Methods Thirty-two SD rats were randomly divided into 4 groups:the control group,low,moderate and high-dose fluoride group.The control group ate commou fodder.The low,moderate and high dose fluoride group were fed with fodder composed of 25%.35%and 68%of corn(containing fluorine of 148.00 mg/kg)in chronic endemic fluorosis region in Guizhou Province.After 140 days,the expressions of BMP-2 and BMP-7 protein were determined by immunohistochemistry and assayed the absorbanee by computer image-pattern analysis system.Light microscope was used to observe the synovial tissue by Hematoxin Eosin,and calculated the pathological integral of synovium according to pathological grade standard.Results The expressions of BMP-2 (32.50±2.73)and BMP-7(38.90±2.56)in the control group was spare.Compared with the control group,the expressions of BMP-2(59.43±5.12,79.82±6.41,101.76±7.56)and BMP-7(55.10±4.82,78.42±5.61,98.46± 6.05)in the synovial tissue was up-regulated in each experimental groups(P＜0.05),especially in the moderate dose and the high-dose groups(P＜0.05).Compared with the control group(0.54±0.21).the pathological integral of synovium increased(P＜0.05)in each experimental groups(1.04±0.98,4.69±1.28,8.60±2.07).The expressions of BMP-2 and BMP-7 in the synovial tissue was found to be positively related with the pathological integral of synovium(r=0.98,0.99,P＜0.05).Conclusion The BMP-2 and BMP-7 play an important role in the development of fluorosis arthritis,probably by affecting osteogenesis.
