Identification of 2 strains of suspected Yersinia pestis isolated from Marmot,,himalayana in Dege County,Sichuan Province
10.3760/cma.j.issn.1000-4955.2009.01.013
- VernacularTitle:四川省德格县2株疑似喜马拉雅旱獭鼠疫菌株的鉴定
- Author:
Zhi-zhen, QI
;
Dan-ba, LUOZHI
;
Yong-jun, DUAN
;
Min, LI
;
Bai-zhong, CUI
;
Rui-xia, DAI
;
Jian-ping, FENG
;
Cun-xiang, LI
;
Shou-hong, YU
;
Zu-yun, WANG
;
Hu, WANG
;
Xing, JIN
;
Hai-hong, ZHAO
;
You-quan, XIN
;
Ling-ling, REN
;
Qing-wen, ZHANG
;
Rong-jie, WEI
;
Li-xia, JIN
;
Yong, JIG
;
Hao-ming, XIONG
;
Xiao-lin, LUO
;
Sang-zhu, ZEREN
;
Hong, WANG
;
Shan-hu, ZHANG
;
Wen-tao, GUO
;
Xue, WANG
;
Ze-li, DANBA
;
Dan, WENG
;
Dai-li, WANG
- Publication Type:Journal Article
- Keywords:
Yersinia pestis;
Biologcal assay;
Marmota hirnalayana;
Plague natural focus
- From:Chinese Journal of Endemiology
2009;28(1):48-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective Throush identify biochemical characteristics and virulence factors of 2 strains suspected Yersinia pestis(Y.pestis)isolated from the dead Marmota himalayana(M.himalayana)to confirm the nature epidemic focus in Dege County,Sichuan Province.Methods Y.pestis was analyzed by specific staining and shape,culturing characteristics,splitting-test by bacteriophage,test of biochemical characteristics and glycolysis ability,virulence factors,virulence,nutritional requirement,plasmid,genetic test and genetic type. Results The tested strains were Gram staining bacilus.The main biochemical characteristics were Arabinose(+)、 Rhamnose(-),Maltose(+),Melibiose(-),Glycerol(+),Denitrification(+).The virulence factors with FI+.VW+, Pgm+,Pst I+;and with the common 6.0×106,45.0×106,65.0×106 plasmids,also with the virulence-relative plasmid gene.Both their absolutely lethal dose(LD100)in mice were 50 bacteria.The nutritional requirement appeared which were depended on Phenylalanine and Methionine.With the Genomovar 5 genotype characteristics of M.himalayana plague foci of Qinghai-Tibet plateau.The difference between tested strains and Yersinia pseudotubercuosis on the 3 different culture medium was obvious.The tested strains had a Y.pestis' specific 3a fragment,Pst I and FI-Ag,at 22 ℃,the strains could be split by bacteriophage completely.Conclusions According to the diagnostic criteria of plague in China,the 2 suspected strains isolated from Dege County,Sichuan Province ale confirmed as Y.pestis.both with powerful virulenceand with the characteristics of the Y.pestis of M.himahtyana in Qinghai-Tibet plateau plague natural focus.
