IGF-I and -II production during menstrual cycle.
- Author:
Jae Yen SONG
1
;
Ji Sun WEE
;
Hyun Jung CHO
;
Ill Young KOOK
;
Hyun Hee JO
;
Mee Ran KIM
;
Dong Jin KWON
;
Chang Suk KANG
;
Jang Heub KIM
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Catholic University of Korea, Korea. janghkim@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
IGF-I;
IGF-II;
Ovarian Follicle;
Menstrual cycle;
Immunohistochemistry
- MeSH:
Corpus Luteum;
Enzyme-Linked Immunosorbent Assay;
Estradiol;
Female;
Humans;
Immunohistochemistry;
Insulin-Like Growth Factor I*;
Insulin-Like Growth Factor II;
Menstrual Cycle*;
Ovarian Follicle;
Ovary
- From:Korean Journal of Obstetrics and Gynecology
2006;49(7):1515-1526
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: We designed this study to understand the physiologic effects and secretory pattern of IGF-I and IGF-II in human serum and changes in expression of IGF-I and IGF-II in human ovarian tissues during menstrual cycle, and to know which one is more important on human ovarian function between IGF-I and IGF-II, related to FSH, LH and estradiol. METHODS: IGF-I, IGF-II, FSH, LH and estradiol levels were measured in 80 serum samples by ELISA from normal reproductive women. We also examined the immunohistochemical staining of the IGF-I and IGF-II in the ovarian tissues of 14 normal reproductive women. The mean age was 35.6+/-9.15 years-old, ranged from 20 to 45. The average menstrual cycle was 27 to 29 days. RESULTS: 1. The average serum concentration of IGF-I was 204.43+/-50.92 ng/mL, and that of IGF-II was 1381.56+/-292.56 ng/mL. 2. The regular pattern or relationship on serum IGF-I and IGF-II concentrations were not observed (P=0.19). 3. To cross-correlation of serum concentrations of FSH, LH, estradiol and IGF-I, IGF-II, IGF-II was thought to effect on human ovarian menstrual cycles, affected by action of FSH (P=0.048). 4. In the normal reproductive ovaries, we observed immunohistochemical staining for IGF-I in primary, secondary, mature follicle, corpus luteum and stroma, but not in corpus albicans. 5. In the normal reproductive ovaries, we observed immunohistochemical staining for IGF-II in primary, secondary, mature follicle, and corpus luteum but not in corpus albicans and stroma. 6. Stronger immunohistochemical staining was observed in ovaries for IGF-II, rather than IGF-I. CONCLUSION: IGF-I and IGF-II were produced by ovarian tissues, and participated in ovarian folliculogenesis according to menstrual cycles by paracrine, autocrine functions. IGF-II, rather than IGF-I, was thought to effect greater on human ovarian menstrual cycles, affected by action of FSH.
