Preparation of a kind of G418 resistance fibroblast feeder layers
- VernacularTitle:G418抗性的小鼠胚胎干细胞滋养层的制备
- Author:
Jiang, ZHOU
;
Xiao, YANG
;
Yan-Xun, SUN
;
Ya-Xin, L*
;
Cui-Fen, HUANG
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(1):59-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare a kind of G418 resistance fibroblast feeder layers.Methods: In order to prepare a stock primary embryonic fibroblast (EMFI) cells, the pregnant Smad3ex8+/- mice containing heterozygous neo gene were killed 14 days after coitus to get the embryo. EMFI cells were tested by PCR to identify their gene types. Only one EMFI cell which contained heterozygous neo gene was chosen and treated with mitomycin C.The mitomycin C-treated EMFI feeders were stored at -70℃. Results: The EMFI feeder layers could support the growth of embryonic stem cell line TC-1 efficiently and could live in G418 resistance medium for 1-2 weeks.
