Etiologic diagnosis and analysis of the first case of human brucellosis in Guizhou province
10.3760/cma.j.issn.1000-4955.2012.06.013
- VernacularTitle:贵州省首例人间布鲁杆菌病病例的病原学诊断与分析
- Author:
Shi-jun, LI
;
Yue, WANG
;
Ding-ming, WANG
;
Ke-cheng, TIAN
;
Ying, LIU
;
Qing, MA
;
Zhao-bin, LIU
;
Xiao-jun, GONG
;
Guang-peng, TANG
;
Gui-chun, CHEN
- Publication Type:Journal Article
- Keywords:
Brucellosis;
Polymerase chain reaction;
Biological assay
- From:Chinese Journal of Endemiology
2012;31(6):643-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To etiologically diagnose and analyze a patient with suspected cases of brucellosis,and to provide a experimental basis for the confirmation of the first case of human brucellosis in Guizhou province.Methods Conventional and molecular techniques [genus specific Brucella surface protein 31 PCR (BCSP31-PCR)and Brucella suis species-specific PCR (AMOS-PCR)] were used to identify suspicious bacteria strains isolated from the suspected patient of brucellosis.Results The results showed that the Brucella suspicious colonies were identified as Brucella melitensis biotype 3 using conventional tests and were further identified as Brucella spp.by genus specific Brucella surface protein 31 PCR (BCSP31-PCR) and classified as Brucella melitensis with Brucella abortus,Brucella melitensis,Brucella ovis,Brucella suis species-specific PCR(AMOS-PCR).Conclusions Laboratory diagnostic results show that the bacteria strain isolated from the suspected patient of brucellosis is Brucella melitensis biotype 3.It is the first case of human brucellosis in Guizhou province.
