Evaluation of the effect of up-converting phosphor technology in detection of plague antigen-antibody by receiver operating characteristic curve method
10.3760/cma.j.issn.1000-4955.2012.06.012
- VernacularTitle:受试者工作特征曲线法评价上转发光技术快速检测鼠疫抗原抗体的效果
- Author:
Jin-xiao, XI
;
Hong, ZHANG
;
Peng-fei, GE
;
Li-min, GUO
;
Wen-ping, DA
;
Bin, WU
;
Da-qin, XU
;
Shi-ming, WANG
;
Wei-min, PAN
;
Ke-jun, MIAO
;
Ya-jun, GE
;
Tao-xia, MU
;
Guo-juan, CHEN
- Publication Type:Journal Article
- Keywords:
Plague;
receiver operating characteristic curve;
Up-conversion technology;
Diagnosis;
Evaluation studies
- From:Chinese Journal of Endemiology
2012;31(6):640-642
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of up-converting phosphor technology(UPT) in detection of plague antigen-antibody by receiver operating characteristic curve (ROC) method,and to provide a scientific basis for field application of UPT rapid detection technology in plague prevention and control.Methods Two hundred and twenty four serum samples were collected from Marmots and ground squirrels in the plague foci,Yersinia pestis antibody was detected by UPT,ELISA,Colloidal-gold Strips and IHA,respectively; 108 organs and bone marrow samples were collected,and Yersinia pestis antigens were detected by UPT,ELISA,PCR and RIHA,respectively.IHA was used as the gold standard for antibody test results,RIHA,PCR + Colloidal-gold Strips,PCR + ELISA were used as the gold standard for antigen test results.The results were evaluated using ROC method.Results Antibodies detection:the AUCs of UPT,ELISA and Colloidal-gold Strips were greater than 0.5.The difference between UPT and other methods was not statistically significant (z =1.204,P ＞ 0.05).Antigen detection:the AUCs of UPT,ELISA,Colloidal-gold Strips and PCR were greater than 0.5.There was no statistical difference between UPT and other methods(z =0.866,P ＞ 0.05).Conclusions UPT as a new technology works well in the detection of plague antigen-antibody.The technology is simple,fast,accurate,and suitable for on-site monitoring of plague,emergency treatment of sudden plague,and suitable for promotion.
