Expression of 8-hydroxy-2-deoxy guanosine, thioredoxin reductase 1 and glutathione peroxidase 1 in myocardium of autopsy patients with Keshan disease
10.3760/cma.j.issn.1000-4955.2012.06.010
- VernacularTitle:克山病尸检患者心肌组织8-羟基脱氧鸟苷酸和硫氧还蛋白还原酶1及谷胱甘肽过氧化物酶1的表达
- Author:
Jun-rui, PEI
;
Ming-fa, LIU
;
Yang, LIU
;
Hong-qi, FENG
;
Zhi-yi, ZHANG
;
Ling-wang, ZHOU
;
Xue-kuan, ZHONG
;
Tong, WANG
- Publication Type:Journal Article
- Keywords:
Keshan disease;
8-hydroxydeoxyguanosine;
Glutathione peroxidase;
Thioredoxin reductase;
Myocardium,human body
- From:Chinese Journal of Endemiology
2012;31(6):631-634
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study,we investigated the relationship between oxidative stress,selenoproteins level and onset of Keshan disease (KD) through detecting the expression of 8-hydroxy-2-deoxy guanosine (8-OH-dG),thioredoxin reductase 1 (TrxR1) and glutathione peroxidase 1 (GPx1) in myocardial tissue.Methods Myocardium samples of autopsy patients including 8 cases of KD (KD group included 4 acute KD and 4 chronic KD) and 9 cases of non-KD (control group) were immunohistochemically stained for 8-OH-dG,TrxR1 and GPx1.The staining intensities subsequently quantified by using Olympus Image-Pro Plus 6.0 software.Results The positive rate of 8-OH-dG expression in myocardial nuclei was higher in the case group[(68.6 ± 20.4)％] than that of the control group[(2.4 ± 1.5)％,t =8.515,P ＜ 0.05].In addition,the positive rate of 8-OH-dG expression in acute KD[(91.7 ± 3.7)％] was significantly higher than that of chronic KD[(53.2 ± 7.9)％,t =6.409,P＜0.05].The distribution of TrxR1 and GPx1 was not associated with the distribution of myocardial damage.The expression of these two selenoproteins in KD group (401340 ± 59865,497590 ± 197082) were both lower than that of control group(2790300 ± 379298,1348400 ±615840; t =-28.493,-6.016,respectively,all P＜0.01).Conclusions Oxidative damage is detected in myocardium tissue of KD,and 8-OH-dG expression is associated with the degree of myocardial damage in KD.Selenoproteins,TrxR1 and GPx1,may be closely related to the pathogenesis of KD.
