Application of multiplex polymerase chain reaction to identify Brucella
10.3760/cma.j.issn.2095-4255.2013.05.007
- VernacularTitle:应用多重聚合酶链式反应鉴别布鲁杆菌
- Author:
Li-Hong, HAN
;
Zhi-Guo, LIU
;
Miao, WANG
;
Ri-Hong, LIU
;
Bu-yun, CUI
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Brucella;
Biological assay;
Evaluation studies
- From:Chinese Journal of Endemiology
2013;32(5):496-499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of multiplex polymerase chain reaction(Multiple-PCR) in identification of Brucella strains.Methods Six standard Brucella strains (Brucella abortus,Brucella melitensis,Brucella suis,Brucella canis,Brucella ovis,Brucella neotomae) were used as positive controls and Escherichia coli O∶157 and Yersinia enterocolitica O∶9 were used as negative controls.A total of 29 Brucella strains were tested.Brucella strains were amplified by BCSP31-PCR and the species of Brucella with positive results were identified with Multiple-PCR method.Results The results of all 29 amplified Brucella strains were positive with BCSP31-PCR.The identified results of all Brucella strains were positive with Multiple-PCR,including 20 strains of Brucella melitensis,5 isolates of Brucella suis,3 strains of Brucella abortus and 1 strain of Brucella canis.Conclusion Multiple-PCR method is a rapid,specific,simple and low risk method for identification of Brucella species.
