Relationship between arsenic (+3 oxidation state) methyltransferase genetic polymorphisms and susceptibility to endemic arsenism
10.3760/cma.j.issn.2095-4255.2013.01.010
- VernacularTitle:三价砷甲基转移酶基因多态性与地方性砷中毒易感性的关系研究
- Author:
Wen-sheng, HOU
;
Li-fang, ZHANG
;
Qiu-ling, PEI
- Publication Type:Journal Article
- Keywords:
Arsenic poisoning;
Arsenic (+3 oxidation state) methyltransferase;
Polymerase chain reaction;
Genes;
Polymorphism,restriction fragment length
- From:Chinese Journal of Endemiology
2013;(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between arsenic(+3 oxidation state) methyltransferase (AS3MT) genetic polymorphism and susceptibility to endemic arsenism.Methods Polymerase chain reactionrestriction fragment length polymorphism-single strand conformation polymorphism(PCR-RFLP-SSCP) technology was performed to detect mutations of AS3MT gene intron 8 and exon 9 in genome DNA of the 79 cases and 110 controls.PCR products with abnormal band forms were further sequenced to find the types and sites of mutation.Chi-square test and multivariate Logistic analyses were conducted.Results The incidence of the 9149 base mutation(A→C) in AS3MT gene intron 8(AS3MT-9149) in case group(19.0％,15/79) was lower than that in control group (23.6％,26/110).The incidence of the codon 287 mutation(ATG→AT/CG) in AS3MT gene exon 9(AS3MT-287)in case group(10.1％,8/79) was lower than that in control group (11.8％,13/110).However,statistical analysis indicated no significant difference in both mutations between two groups[AS3MT-9149:odds ratio(OR) =0.59,95％ confidence interval(CI):0.26-1.31,P =0.195; AS3MT-287:OR =0.85,95％ CI:0.32-230,P =0.751].Conclusions There are no significant association between the genetic polymorphisms of AS3MT-9149,AS3MT-287 and the susceptibility to endemic arsenism.Similarly,due to small sample amount,we can not exclude the possibility that these gene polymorphisms are related to susceptibility to endemic arsenism.
