Comparion of the expression of CS3 fimbriae in different vector systems
- VernacularTitle:CS3菌毛在不同载体系统中表达水平的比较
- Author:
Rong-Kai, GAO
;
Zhao-shan, ZHANG
;
Shu-Qin, LI
;
Cui-Fen, HUANG
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To choose the best vector for the expression of CS3 fimbriae. Methods: The CS3 operon was cloned into different plasmid vectors such as pUC19 and pTrc99A. The expression of CS3 was monitored by whole-cell ELISA and SDS-PAGE analysis. The assembly of CS3 fimbriae was detected with electron microscopy. Results:The expression level of CS3 fimbriae using plasmid pUC19 as carrying vector was the highest, and the insertion orientation of CS3 gene into the plasmid has a little effect on its expression level. The expression of CS3 fimbriae was confirmed by SDS-PAGE analysis and electron microscopy.Conclusions:The promotor of CS3 itself played the key role in the expression of CS3 fimbriae and the copy number of plasmid was the main factor to affect the expression level.
