Cloning and expression of dengue 2 virus NS1 gene in eukaryotic cells
- VernacularTitle:登革2型病毒43株NS1基因的克隆及在真核细胞中的表达
- Author:
Zhi-jun, HU
;
Jing, YANG
;
Wei, ZHAO
;
Pei-ying, YANG
;
E-De, QIN
;
Bao-Chang, FAN
;
Li-qing, GENG
;
Man, YU
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective：To investigate the correct expression of dengue 2 virus 43 strain NS1 gene in transfected BHK-21 cell. Methods：The D2-43 DNA fragment coding for signal peptide plus NS1 protein was cloned between KpnⅠ site and EcoR Ⅰ site of expression plamid pcDNA3.1. The obtained recombinant vector pcDNA-NS1 was transfected into BHK-21 cells with electroporation technique. After selection by G418, resistant clones were screened by RT-PCR and Western blotting test. Results：The RT-PCR results of four in five randomly selected cell clones were positive. Western blotting test showed that NS1 gene could be expressed in BHK-21 cells. Conclusions：NS1 protein was capable of being expressed and appropriately processed in pcDNA-NS1 transfected BHK-21 cells. The present results suggest the feasibility of NS1-based DNA immunization.
