Effect of T-2 toxin on apoptosis of fetus chondrocytes
- VernacularTitle:T-2毒素致胎儿软骨细胞凋亡的观察
- Author:
Tian-fu, YANG
;
Zhi-qiang, JIA
;
Bin, SHEN
- Publication Type:Journal Article
- From:Chinese Journal of Endemiology
2001;20(2):84-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of T-2 toxin on apoptosis of chondrocytes.Methods Chondrocytes which were obtained from aborted fetal were cultured in vitro.Four days later,these chondrocytes were exposed to T-2 toxin in different concetrations for 16 hours.According to the concentratio ns,five experimental groups were divided:0,5,10,20,40 μg/L.Then TUNEL staining and Flowcytometry were used to detect the apoptosis of chondrocytes qualitativel y and quantitatively,the effect of T-2 toxin on proliferation of chondrocytes were also observed.Results After being exposed to T-2 toxin,the body of chondrocytes shrinked obviously and there was a dose-dependent relationship bet ween the toxin concentration and the degree of shrink.The concentration of T-2 toxin changed from 0 μg/L to 10 ng/ml,the number of apoptosis increased.Conclusions T-2 toxin can inhibit the proliferation of chondroyte significantly in a dose-depenent manner. T-2 toxin can induce the apoptosis of chondrocyte and the numbers of apoptosis is proportionate to the concentration of T-2 toxin in particular range.
