Construction of a Saccharopolyspora erythraea mutant producing erythromycin B as its major fermentation product
- VernacularTitle:产红霉素B红色糖多孢菌突变体的构建
- Author:
Hao, ZHAO
;
Xiang, DONG
;
Bu-Chang, ZHANG
;
Hua, YUAN
;
Xun-Duan, HUANG
;
Shu-Xiang, ZHANG
- Publication Type:Journal Article
- Keywords:
Cytochrome P450 monooxygenase;
Saccharopolyspora erythraea;
eryK
- From:Bulletin of The Academy of Military Medical Sciences
2009;33(1):18-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain a large amount of erythromycin B and to investigate the activity site in eryK. Methods:The key sequence of the BC loop region in eryK gene was knocked out and the eryK gene with 101 bp deleted was amplified by overlapping PCR,and cloned into vector pWHM3 to construct recombinant plasmid. The Saccharopolyspora erythraea mutant AK17 was constructed through chromosomal homologous recombination technique.Results and Conclusions:The S.erythraea mutant AK17 was constructed. The results of TCL and MS analysis showed that the major fermentation product of AK17 is erythromycin B.
