Progress in research on multilocus sequence typing technique
- VernacularTitle:多位点序列分型技术及其研究进展
- Author:
Zhong-qiang, WANG
;
Shao-fu, QIU
;
Yong, WANG
;
Yan-song, SUN
;
Hong-bin, SONG
- Publication Type:Journal Article
- Keywords:
multilocus sequence typing;
genotyping;
housekeeping gene
- From:Bulletin of The Academy of Military Medical Sciences
2010;34(1):76-79
- CountryChina
- Language:Chinese
-
Abstract:
Multilocus sequence typing (MLST) is a molecular genotyping method based on nucleotide sequencing. The procedure of this method characterizes isolates of bacterial species using the DNA sequencing of multiple housekeeping genes(usually seven). For each housekeeping gene, the different sequences present within a bacterial species are assigned as distinct alleles.For each isolate, the alleles at each of the loci define the allelic profile or sequence type (ST). MLST has the advantages of being robust (based on genetic data) and electronically portable to generate data that allow rapid and global comparisons between different laboratories. In this paper, the principle, method, data analysis, application, advantages and flaws of MLST are introduced.
