Analysis on Hantaan virus in hemorrhagic fever patients with renal syndrome in Heilongjiang
- VernacularTitle:黑龙江省肾综合征出血热患者血清汉坦病毒致病株分析
- Author:
Jun-ying, DAI
;
Ming-rong, LI
;
Jian-wu, YU
;
Chun-hai, XU
;
Shu-chen, LI
;
Yu-guang, ZHAO
- Publication Type:Journal Article
- Keywords:
Hemorrhagic fever with renal syndrome;
Hantaan virus;
Sequence analysis
- From:Chinese Journal of Endemiology
2008;27(3):341-343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To separate and amplify Hantaan virus(HV)in serum of hemorrhagic fever patients with renal syndrome(HFRS)in Heilongjiang,and look for its difference from intemational standard type strain(76-118strains).Methods HVs of different phase in the 8erum of 50 HFRS patients were separated and amplified by RTnested-PCR,its products were analyzed the amplified by sequencing.Results Detectable rate of HV in the patients serum was 36.36%(8/22)in 7 days after onset,it Was 13.04%(3/23)in patients having an onset 8 days to 14 days earlier,5 cases were not detectable 15 days after onset.Comparing the sequence of HV S gene fragment,sample 1,9,18,31,37,38,44 strain had a homology of 90.24%,86.72%,89.97%,89.16%,86.45%,87.26%and 89.43%to 76-118 strains,respectively.Conclusions The positive rate is the highset in 7 days after onset.Nucleotide sequence difference exists between pathogenic strain of Heilongjiang's HV and international standard strain,indicating that not only hosts but also locations can affect HV.
