Genetics comparison on the Yersinia pestis strains of 3 kinds of ecotypes in the Sanjiangyuan area, Qinghai Province
- VernacularTitle:青海省三江源地区3种生态型鼠疫耶尔森菌的基因多态性比较
- Author:
Bai-zhong, CUI
- Publication Type:Journal Article
- Keywords:
Random amplified polymorphic DNA technique;
Yersinia pestis;
Ecotype
- From:Chinese Journal of Endemiology
2008;27(5):508-510
- CountryChina
- Language:Chinese
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Abstract:
Objective To compare the genetic polymorphism of 34 Yersinia pest strains of 3 kinds of ecotypes(Microtus fiscis,Qilian Mountain and Qinghai-Tibet Plateau ecotype)that isolated from the Sanjiangyuan area in Qinghai Province and Shiqu County in Sichuan Province,in order to know the ecotype relationship among the strains.Methods Thirty-four strains were amplified using the application of random amplified polymorphism DNA(RAPD)and were detected with agaroge gel electmphoresis.Results Amplified products by ngarose gel electrophoresis method showed same stripes in 31 strains,only 3 strains had slight differences.Conclusion The Microtus fuscas,Qilian Mountain and Qinghai-Tibet Plateau ecotype strains isolated from Sanjiangyuan area are genetically homologous.
