Study on the Yersinia pestis plasmid along the Qinghai-Tibet Railway
- VernacularTitle:青藏铁路沿线鼠疫菌质粒的研究
- Author:
Chun-hua, ZHANG
;
Jing-sheng, L(U)
;
Bin, ZHAO
;
Xian-bin, CONG
;
Zhong-hui, WANG
;
Shi, ZHANG
;
Kui-dong, SHAO
- Publication Type:Journal Article
- Keywords:
Yersiniapestis;
Plasmids;
Electrophoresis,agar gel
- From:Chinese Journal of Endemiology
2008;27(6):595-597
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the Yersinia pestis plasmid and molecular weight along the Qinghai-Tibet Railway.Methods Yersinia pestis plasmids molecular weight detected and analyzed using alkaline lysis,phenol-chloroform extraction of Yersinia pestis plasmid by agarose gel electrophoresis.Results The 18 Yersinia pestis strains of Qinghai-Tibet Railway contained 6×106,45×106,52×106,65×106,92×106plasmid,varing in the range of the 52×106-92×106.Conclusions The Yersinia pestis of Qinghai-Tibet Railway has a standardplasmid graphics,with the biggest Yersinia pestis plasmid changing in a certain regular degree,which providessignificance in the study of plague natural foci of the spatial structure and the genetic.characteristics of Yersiniapestis.
