Screening of differential serum proteins in patients with hepatic injury resulting from coal-burning type of arsenism
10.3760/cma.j.issn.1000-4955.2010.06.004
- VernacularTitle:燃煤污染型砷中毒肝损伤患者血清差异蛋白质的筛选
- Author:
Ru-jia, XIE
;
Bing, HAN
;
Xin-hua, LUO
;
Jun, WU
;
Ming-liang, CHENG
;
Qin, YANG
- Publication Type:Journal Article
- Keywords:
Arsenic poisoning;
Liver injury;
Serum;
Two-dimensional gel electrophoresis;
Proteome
- From:Chinese Journal of Endemiology
2010;29(6):604-607
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the differential serum proteins in patients with hepatic injury resulting from coal-burning type of arsenism. Methods Six serum samples were collected from patients with liver injury resulting from coal-burning type of arsenism and healthy subjects(control gruop) in endemic arsenism area. Twodimensional gel electrophoresis(2-DE) was performed to separate serum proteins, after silver staining, the differential expression of proteins were analyzed and then identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS). Results The 2-DE map of serum protein patterns of patients and normal control were established successfully. The results showed that there were an average of (824 ± 31 ) spots and (782 ± 42) spots on 2-DE matching of the patients and control groups and the matching rate was 94.9%(782/824). From these two groups 49 differential protein spots were identified, of which over 3 times the difference in the expression of 30 protein spots were singled out and MALDI-TOF-MS analysis was carried out. Ten proteins were identified. Upregulated expression was observed in alpha-2-macroglobulin, B-cell receptor-associated protein, keratin 1,apolipoprotein A-I, and down-regulated expression was observed in haptoglobin, α2-heremans-schimid-glycoprotein,mitogen-activated protein kinase 4, zinc finger protein 323, ZAP-70 and SP40 in the patient group. Conclusions The well-resolved and reproducible 2-DE serum patterns of patients are established and some differentially expressed proteins are characterized. Whether these proteins of differential expression are serum markers for liver injury resulting from coal-burning type of arsenism need to be further verified.
