Separation and purification of catalpol from leaves of Rehmannia by macroporous adsorption resins:a priliminary study
- VernacularTitle:大孔吸附树脂分离纯化鲜地黄叶中梓醇的初步研究
- Author:
Yan-Wei, KUANG
;
Yan-Wei, LU
;
Chu-ze, WU
- Publication Type:Journal Article
- Keywords:
Rehmannia;
catalpol;
macroporous adsorption resin;
separation and purification
- From:Bulletin of The Academy of Military Medical Sciences
2009;33(6):550-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain the optimal conditions for separating catalpol from leaves of Rehmannia by selecting appropriate macroporous adsorption resins.Methods:The detection indication was the content of catalpol, which was determined by HPLC method. Twelve different kinds of macroporous adsorption resins were studied on the static capacity of adsorption and desorption, and H103 resin was selected for the research of separation and purification.Results:The H103 resin had a good capacity for adsorption and desorption.The best process of purifying catalpol by H103 resin was 1mg/ml concentration, the adsorption rate of 1-2 BV/h,the flow rate of 1-3 BV/h, and 8 BV with 10% alcohol.Conclusion:The method is simple and available, which can simplify the production process and lower costs.
