Quantitative determination of metoprolol by liquid chromatography mass spectrometry in rat plasma
- VernacularTitle:LC-MS法检测大鼠血浆中酒石酸美托洛尔的方法学研究
- Author:
Fan, ZHANG
;
Meng-Xun, CUI
;
Zhen-Qing, ZHANG
- Publication Type:Journal Article
- Keywords:
metoprolol;
pharmacokinetics;
LC-MS
- From:Bulletin of The Academy of Military Medical Sciences
2009;33(6):543-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a highly sensitive, rapid and selective liquid chromatography mass spectrometry (LC-MS) method for the determination of metoprolol in rat plasma.Methods:A simplified liquid-liquid extraction with acetidin was employed for the sample preparation. The separation was carried out on a Thermo ODS-C_(18)(5 μm,100 mm×2.1 mm).The mobile phase consisted of acetonitrile-methanol-water(20∶20∶60). Propranolol was used as the internal standard. The detection was performed on a liquid chromatography mass spectrometry by selected ion monitoring(SIM) scan mode electrospray ionization(ESI).Results and Conclusion:The range of calibration curve was 0.1-50 ng/ml and the limit of quantification was 0.1 ng/ml. The intra- and inter-day precision RSD was less than 15%.This method is sensitive, simple,rapid and suitable for the pharmacokinetic study of metoprolol.
