Rapid and quantitative detection of abrin using colloid gold immunochromatographic assay
- VernacularTitle:胶体金免疫层析技术快速定量检测相思子毒素
- Author:
Cong, NIE
;
Jing, WANG
;
Xiao-Hong, SUN
;
Shan, GAO
;
Yuan-Hua, YU
;
Lin, KANG
;
Jing-Lin, WANG
- Publication Type:Journal Article
- Keywords:
abrin;
colloid gold;
immunochromatographic assay;
quantitative detection
- From:Bulletin of The Academy of Military Medical Sciences
2009;33(6):546-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a rapid and quantitative detection method for abrin using colloid gold immunochromatographic assay. Methods:The rapid detection method was established with double-antibody sandwich assay. Quantification was realized by constructing a standard curve. The specificity and sensitivity of the method were tested, and its feasibility was evaluated by various abrin-added food samples. Results and Conclusion:This established method could accomplish qualitative and semi-quantitative detection in 15 minutes; the sensitivity was up to 30 ng/ml with a linear range from 30 ng/ml to 600 ng/ml. The recovery rate was 80%-110%, and the variation coefficient was less than 15%.The colloidal gold immunochromatographic assay is rapid, specific, sensitive,accurate and suitable for field detection.
