Improvement of rat pulmonary microvascular endothelial cell primary culture methods
- VernacularTitle:大鼠肺微血管内皮细胞原代培养方法的改进
- Author:
Ge, MENG
;
Jian, ZHAO
;
Xin-Huai, L(U)
;
Ri-Gao, DING
- Publication Type:Journal Article
- Keywords:
pulmonary microvascular endothelial cells;
isolation;
culture;
identification
- From:Bulletin of The Academy of Military Medical Sciences
2009;33(6):567-569
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the primary culture method of rat pulmonary microvascular endothelial cells(PMVECs) and obtain purified PMVECs.Methods:The modified tissue block pasted culture method was used to isolate and culture Wistar rat PMVECs. The morphous of cultured cells were observed by microscopy. The cultured cells were identified by detecting factor Ⅷ related antigen and binding isolectin B4. Results and Conclusion:The morphous of cultured primary PMVECs in vitro showed short fusiform shape or polygon, and the monolayer of cultured cells displayed the shape of pavingstone. But the morphous changed followed the transfer of culture and the change of culture condition. The cultured cells had characterization of binding isolectin B4 and negative immunocytochemical staining for factor Ⅷ related antigen. The cultured PMVECs have good growth state and purity,and can be subcultringed stably.The observation of cell morphous integrating with immunocytochemical staining is a reasonable identification method for PMVECs.
