The effects of amnion on expression of EGFR in rabbit M(u)ller cells
- VernacularTitle:羊膜对体外培养的兔视网膜Müller细胞表皮生长因子受体表达的影响
- Author:
Yuhua, HAO
;
Jingxue, MA
- Publication Type:Journal Article
- Keywords:
amnion;
retinal glial cells;
epidermal growth factor receptor
- From:Chinese Ophthalmic Research
2009;27(12):1086-1088
- CountryChina
- Language:Chinese
-
Abstract:
Objective hole.Research demonstrated that amnion promotes the proliferation of and its mechanism.Methodswhite rabbit.The cells were primarily cultured and passaged in DM/F12 with 10% fetal bovine serum and identified with glial fibrillary acidic using 0.25% trypsin and 0.05%EDTA and suspended in 10% fetal bovine serum+DMEM/F12.0.5 mL of the amnion supernatant was added to the medium for 12 hours.The expression of EGFR in the cells was detected by immunohistochemistry.The difference of EGFR expression with or without the amnion was evaluated.ResultsCultured cells showed a positive response for GFAP and S-100.The intermediate filaments with a length of 8-10 nm were exhibited under the transmission electron microscope.EGFR was fainly expressed in cultured hours,the expression of EGFR was obviously increased with a significant difference in the gray scale between the two groups(571 588.80±67 862.68 vs.1 000 352.00±98 386.22)(t=4.035,P＜0.01).Conclusion
