Effects of Ang-Ⅱ on c-fos mRNA and pro-α1ⅠmRNA of cadiac FBC in rat embryo
- VernacularTitle:血管紧张素-Ⅱ对鼠胚心纤维母细胞癌基因和前胶原基因的影响
- Author:
Yi, JIN
;
Bao-yu, LI
;
Cheng-hao, GUO
- Publication Type:Journal Article
- From:Chinese Journal of Endemiology
2001;20(3):169-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Ang-Ⅱ on expressions of c-fos mRNA and pro-α1［Ⅰ］mRNA in fibroblasts (FBC) of rat embryo in the course of collagen synthesis.Methods The change of free Ca2+(［Ca2+］i) was tested in fluorometry and the expressions of c-fos mRNA and pro-α1［Ⅰ］mRNA were tested by RT-PCR method in incubated FBC.Results The level of［Ca2+］i in FBC and the expressions of c-fos mRNA and pro-α1［Ⅰ］mRNA were enhanced with the increase of Ang-Ⅱ concentration(10-8～10-5and 10-7～10-6mol/L).Conclusions The expressions of c-fos mRNA and pro-α1［Ⅰ］mRNA were induced by Ang-Ⅱ activating signal system in FBC.
