Using the Fluorescence in Situ Hybridization technique for prenatal diagnosis of Down’s syndrome
- Author:
Tran Thi Thanh Huong
- Publication Type:Journal Article
- Keywords:
Down’s syndrome
- MeSH:
Down Syndrome;
Prenatal Diagnosis
- From:Journal of Practical Medicine
2005;530(11):9-11
- CountryViet Nam
- Language:Vietnamese
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Abstract:
Fluorescence in Situ Hybridization (FISH) is cytogenetics - molecular technique. FISH is the rapid detection technique of chromosomal aberration by chromosome specific probes applied to interphase cells. In this study, the authors used Kit duple DNA probe for chromosome 13, 21, X, Y to prenatal diagnosis of Down’s syndrome. With 6 amniotic fluid samples, the authors obtained the results as follows: 2 samples were Down’s syndrome trisomy; 1 sample was Patau syndrome trisomy; 3 samples were normal; 1 sample male foetus died without clear causes. Cytogenetic result: 46, XX but FISH result: 46, XY; PCR (Polymerase Chain Reaction) result: TDF (Testis Determining Factor): (+). These results were confirmed by chrosome analysis.
