Anti-angiogenic effect of the transmemberane protein(Tenomodulin) on retinal neovascularization in a mouse model
10.3969/j.issn.1003-0808.2010.02.005
- VernacularTitle:Tenomodulin抑制氧诱导视网膜病变小鼠模型新生血管形成的研究
- Author:
Wang, WEI
;
Oshima, YUSUKE
;
Tano, YASUO
- Publication Type:Journal Article
- Keywords:
tenomodulin protein;
retinal neovascularization;
C57BL/6J mouse
- From:Chinese Ophthalmic Research
2010;28(2):114-118
- CountryChina
- Language:Chinese
-
Abstract:
Background Retinal vascular diseases is a main causing-blindness eye disease.Tenomodulin(TeM) is proved to be an inhibitor of neovascularization.But its mechanism is still below understanding.Objective The present paper is to explore the anti-angiogenic effect of TeM protein on retinal neovascularization in a mouse model of retinopathy of prematurity(ROP).Methods Sixty 7-day-old C57BL/6J mice were used in this study.Fifteen mice were exposed to(75±2)% oxygen for 5 days followed by 5 days in room air to create ROP models.Fifteen mice were raised in room air environment from birth through postnatal day 17 as normal groups.1μg TeM was intravitreally injected in lateral eyes and PBS was injected in the fellow eyes and then exposed to(75±2)% oxygen in 30 mice.Eyeballs were obtained at postnatal day 17.Retinal blood vessel patterns were visualized by fluorescein angiography.The number of neovascular nuclei breaking the inner limiting membrane(ILM) was counted with a masked approach,and the inhibitory effects of TeM on retinal neovascularization was observed.The morphology of retina was examined under the light microscope to evaluate the possible toxicity of TeM protein on retina.Western blot analysis was performed to detect the expression of TeM protein in retinal tissue.Results The central retinal nonperfusion area in ROP mouse was(2.94±0.55) mm~2 and that of PBS-injected mouse was(2.83±0.46) mm~2,showing a significant difference in comparison with TeM-injected mouse(0.44±0.26 mm~2)(P＜0.01).The number of endothelial cells nuclei of new blood vessels breaking through inner limiting membrane(ILM) on each retinal cross-section was(10.57±2.95) mm~2 in TeM-injected mouse,showing a significant different with that of ROP eye(44.93±6.78) mm~2 and PBS-injected eye(41.07±7.31) mm~2(P＜0.01).No obvious alteration of morphology in retinal section was found in TeM injected eye.Western blot assay showed an immunoreactive band of TeM with the molecular weight of 16000.No positive expression was detected in PBS injected eye.Conclusion TeM can effectively inhibit pathological angiogenesis in C57BL/6J mouse.This result indicates a potential role of TeM in preventing and treating ocular neovascularization.
