Oxidative damage induced by sodium arsenite in SV-40-immortalized normal uroepithelial cells
10.3760/cma.j.issn.1000-4955.2012.01.004
- VernacularTitle:亚砷酸钠诱导人膀胱上皮永生化细胞氧化损伤作用观察
- Author:
Sheng-nan, LIU
;
Fei, WANG
;
Hui-hui, WANG
;
Shu-hua, XI
;
Gui-fan, SUN
- Publication Type:Journal Article
- Keywords:
Arsenic poisoning;
Arsenites;
Epithelial cells,urinary bladder;
Wounds and injuries,oxidation-reduction
- From:Chinese Journal of Endemiology
2012;31(1):13-15
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the state of oxidative injury induced by sodium arsenite(NaAsO2) in SV-40-immortalized normal uroepithelial (SV-HUC-1 ) cells.Methods SV-HUC-1 cells were exposed to different concentrations of NaAsO2[0(control),1,2,4,8,10 μmol/L] for 24 h,intracellular reactive oxygen species (ROS) was determined by flow cytometry,and the content ofintracellular nitrotyrosine(NT) and the 8-Hydroxydeoxyguanosine (8-OHdG) levels of cell culture medium were detected by enzyme linked immunosorbent assay (ELISA).Results After 24 h treatment,ROS levels(81.76 ± 4.91,95.23 ± 2.17,126.61 ± 17.95,126.74 ± 27.77,114.18 ± 9.65) of SV-HUC-1 cells in the 1,2,4,8,10 μmol/L NaAsO2 exposure groups were significantly higher than those of the control group (69.84 ± 1.28,P ＜ 0.05 or ＜ 0.01 ),ROS levels and exposure dose were positively correlated significantly(r =0.818,P＜ 0.01); the content of NT in the 10 μmol/L NaAsO2 exposure group[(919.66 ± 206.33) μg/L] was significantly higher than that in the control group[ (238.19 ± 38.28)μg/L,P ＜ 0.01 ],NT content and dye concentrations of arsenic also had dose-response relationship (r =0.617,P ＜ 0.01); after 24 h the cells were treated with arsenic,no significant difference of 8-OHdG content in the culture medium was observed(F =2.127,P ＞ 0.05 ).ConclusionNaAsO2 can cause SV-HUC-1 cell oxidative damage.
