Gene polymorphisms and distribution features of glutathione S-transferase Omega-1 gene in Ala140Asp site in 16 Chinese populations
10.3760/cma.j.issn.1000-4955.2012.01.005
- VernacularTitle:我国16个人群谷胱甘肽硫转移酶GSTO1基因Ala140Asp位点的多态分布特征
- Author:
Song-bo, FU
;
Jie, WU
;
Dian-jun, SUN
- Publication Type:Journal Article
- Keywords:
Glutathione S-transferases class Omega-1;
Genetic polymorphism;
Genetics population
- From:Chinese Journal of Endemiology
2012;31(1):16-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate gene polymorphisms and distribution features of glutathione Stransferase Omega-1 (GSTO 1 ) gene in Ala 140Asp site in 16 Chinese populations.MethodsA total of 1369 samples were from the human genome project(HGP)-the establishment and preservation program of Chinese minority genetic resources.The phenotypes of Ala/Ala (C/C),Ala/Asp (C/A),and Asp/Asp (A/A) of GSTO1 Ala140Asp were determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).With analysis of molecular variance(AMOVA),the genetic variation levels among nations and regions were analyzed by estimating the evolutionary distance of alleles or genotypes.ResultsOf the 1369 individuals analyzed,979 (71.51%) were carriers of the wild homozygous allele Ala/Ala(C/C),365 (26.66%) were heterozygotes Ala/Asp(C/A) and 25 (1.83%) were mutant homozygotes Asp/Asp(A/A),with an overall frequency of the GSTO 1 mutant allele A 15.16% [ (365 +50)/( 1369 × 2)].AMOVA analysis showed that the difference was statistically significant(P < 0.05) of genetic variations of GSTO1 gene Ala140Asp among the 14 ethnic groups,and was significant between the northem and southern populations (P < 0.05 ).Conclusion In different regions and populations the GSTO1Ala140Asp mutant allele frequencies are different.