Increased IL-12 , but Depressed IL-18 Production after In Vitro Stimulation with a 30-kDa Mycobacterial Antigen in Tuberculous Pleural Mononuclear Cells.
- Author:
Chang Hwa SONG
;
Eun Kyeong JO
;
Seong Ho KIM
;
Hwa Jung KIM
;
Ji Won SUHR
;
Tae Hyun PAIK
;
Hyun Hee NAM
;
Jae Hyun LIM
;
Un Ok KIM
;
Ji Sook LEE
;
Jeong Kyu PARK
- Publication Type:In Vitro ; Original Article
- Keywords:
Mycobacterium tuberculosis;
Interleukin-12;
Interleukin-18;
30-kDa antigen;
Tuberculous pleurisy
- MeSH:
Hot Temperature;
Humans;
Interleukin-12*;
Interleukin-18*;
Interleukins;
Mycobacterium tuberculosis;
Pleurisy;
RNA, Messenger;
Trypsin;
Tuberculosis, Pleural
- From:Journal of Bacteriology and Virology
2001;31(3):239-248
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, we investigated interleukin (IL)-18 and IL-12 following in vitro stimulation with either the 30-kDa or purified protein derivative (PPD) antigens (Ag) of pleural mononuclear cells from 12 cases of tubercular pleurisy (TB-PMC) and 8 cases of malignant pleurisy (MG-PMC). Ag-stimulated TB-PMC produced significantly more IL-12 than did MG-PMC and the levels correlated with those of IFN - gamma. Although elevated IL-18 levels were found in freshly isolated pleural fluids, in vitro IL-18 production in response to either Ag was dramatically decreased in TB-PMC. Pro-IL-18 mRNA was detected before and after Ag stimulation in TB patients. Supernatants from the Ag-stimulated TB-PMC significantly suppressed IL-18 production in normal peripheral blood mononuclear cells (PBMC) and primary malignant cells over an 18 h incubation period. In addition, this suppressive activity was not inactivated by either heat or trypsin. Our findings imply that modulation of IL-12 and IL-18 levels may contribute to the Th1 elevation induced in human TB-P VIC by the 30-kDa and PPD antigens.
