Prokaryotic expression and polyclonal antiserum preparation of Schistosoma japonicum Mago nashi like protein
- VernacularTitle:日本血吸虫Mago nashi基因的表达及其抗体的制备
- Author:
Zhi-rong, ZHAO
;
Li, LEI
;
Ji-jia, SHEN
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Gene Expression;
Cloning;
Antibody
- From:Chinese Journal of Endemiology
2008;27(2):158-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express Schistosoma japonicum Mago nashi(SjMago)gene,and prepare its specific polyclonal antibody.Methods SjMago gene was amplified by PCR from Schistosomulum cDNA library and subcloned into pET28a(+)vector,its recombinant proteins were expressed with IPTG.Rabbits were immunized with the polyacrylamide gel particles containing the recombinant proteins for polyclonal antibody preparation,the sera were detected for antibody specificity by Western blot and titer by ELISA assay.Results SjMago prokaryotic expression plasmid was successfully recombined and the target proteins was induced by IPTG in a molecular weight of 17 X 103，the high titer(1∶40 960)polyclonal antibody was isolated from the immunized rabbit，specific rotein band was detected by Western blot.Conclusion SjMago protein has been successfully expressed and its specific polyantibody is prepared,which lays the foundation for further study.
