Effect of subchronic exposure to fluoride on mRNA expression of estrogen receptor in female mice
10.3760/cma.j.issn.2095-4255.2013.02.008
- VernacularTitle:亚慢性染氟对雌性小鼠雌激素受体mRNA表达的影响
- Author:
Cai-sheng, WANG
;
Yong, TANG
;
Chun, WANG
- Publication Type:Journal Article
- Keywords:
Sodium fluoride;
Mice;
Estrogen receptor
- From:Chinese Journal of Endemiology
2013;(2):146-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the subchronic toxicity of sodium fluoride on mRNA expression of estrogen receptor(ER) in female mice.Methods Forty female mice were randomly divided into 4 groups according to body mass,10 in each group,and exposed to sodium fluoride solution(0,1,5,25 mg/L) through drinking water in control,low-dose,medium-dose and high-dose groups for 12 weeks.The expression levels of ERα,ER3 mRNA in ovarian tissues were determined by RT-PCR method.Results The relative expression levels of ERα,ERβ mRNA in control,low-,medium-and high-dose groups were 0.7028 + 0.0474,0.7195 ± 0.0552,0.6479 ± 0.0590,0.5684 ± 0.0513 and 0.8418 ± 0.0131,0.7729 ± 0.0974,0.7610 ± 0.0984,0.8026 ± 0.0234,respectively.The difference between high-dose and control groups of the expression level of ERα was statistically significant (P ＜ 0.05).Conclusions The subchronic toxicity of fluoride can decrease the expression of estrogen receptor in ovarian tissue,which may have a certain effect on reproductive development of female mice.
