In vitro selection of DNA aptamers to Bacillus anthracis spores by SELEX
- VernacularTitle:SELEX法筛选炭疽芽孢杆菌芽孢适配子的研究
- Author:
Bei, ZHEN
;
yajun, SONG
;
Zhaobiao GUO
;
Jin, WANG
;
Minli, ZHANG
;
ShouYi, YU
;
Ruifu, YANG
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(2):111-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain oligonucleotide aptamers which can specifically bind to Bacillus anthracis spores by in vitro selection protocol-SELEX (system evolution of ligands by exponential enrichment).Methods:An in vitro synthesized 78 mer random DNA library (≤1014-15types of different DNAs ) was subjected to 15 rounds of selection using SELEX method against spores of B.anthracis vaccine strain A.16R. Binding of the aptamers to spores was visualized by biotin-streptavidin-horseradish peroxidase system.Results:PCR amplification band pattern of the first round selection was different from that of the ninth round. The binding assay demonstrated that D absorbance at 450 nm of the fifteenth round pool increased 9 times as compared with that of the first round , and the D absorbance increased with the increment of aptamers′ quantity binding to spores. Conclusions: A set of aptamers with considerable binding affinity to B.anthracis spores were successfully selected from the initial random DNA pool.
