Effects of free fatty acid on gene expression and protein tyrosine phosphorylation of leptin-receptor in rats
- VernacularTitle:游离脂肪酸对大鼠瘦素受体基因表达及蛋白酪氨酸磷酸化的影响
- Author:
QiYu, GUO
;
Yan, GAO
;
Lin, CONG
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(2):115-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of free fatty acid on leptin receptor in rats.Methods:High plasma free fatty acid rat model was created by intravenous injection of free fatty acid. Western blot was used to assess the protein level of leptin-receptor in rat liver, muscle and pancreas tissues. The RNA expression level of leptin-receptor was examined by RT-PCR .The tyrosine phosphorylation of leptin-receptor was examined by immuno-precipitation. Results:The protein and RNA levels of leptin-receptor in liver,muscle and pancreas tissues of high plasma free fatty acid rats were similar to those of normal group(P>0.05).But the tyrosine phosphorylation of leptin-receptor significantly downregulated in liver and muscle tissues as compared with control group(P<0.05). Conclusions:High plasma free fatty acid may affect the tyrosine phosphorylation of leptin-receptor in rat liver and muscle.
