Effects of chronic arsenic exposure on estrogen receptor-binding fragment-associated gene 9 and estrogen-responsive finger protein mRNA expression in female rat's myocardium
10.3760/cma.j.issn.2095-4255.2013.01.007
- VernacularTitle:慢性砷暴露对雌性大鼠心肌雌激素受体结合位点相关抗原9和雌激素反应指蛋白mRNA表达的影响
- Author:
Zhi-wei, GUO
;
Wei-hong, YANG
;
Xiao-hong, HAN
;
Ya-juan, XIA
- Publication Type:Journal Article
- Keywords:
Arsenic;
Myocardium;
Estrogen receptor-binding fragment-associated gene 9;
Estrogen-responsive finger protein
- From:Chinese Journal of Endemiology
2013;(1):24-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of chronic arsenic exposure on estrogen receptor-binding fragment-associated gene 9 (Ebag9) and estrogen-responsive finger protein (efp) mRNA expression in female rat' s myocardium.Methods Fifty female Wistar rats were randomly divided into five groups according to arsenic (As2O3) concentrations in drinking-water:0.00(control),0.05,0.10,0.20,0.40 mg/L groups and RT-PCR was used to detect Ebag9 and efp mRNA expression of myocardium at the 32 weeks of experiment.Results Ebag9 and efp mRNA expression levels in 0.00,0.05,0.10,0.20,0.40 mg/L groups were respectively as follows:0.54 ±0.14,0.52 ± 0.10,0.48 ± 0.24,0.58 ± 0.13,0.45 ± 0.19 and 0.85 ± 0.14,0.86 ± 0.12,0.87 ± 0.09,0.99 ±0.10,0.86 ± 0.19.Compared to the control group,Ebag9 mRNA level of the 0.20 mg/L group was increased,and decreased in other groups,but the difference between two groups was not significant(all P ＞ 0.05).Compared to control group,the efp mRNA level of 0.20 mg/L group increased significantly(P ＜ 0.05),and showed increased tendency in other arsenic groups,but the difference between two groups was not significant (all P ＞ 0.05).Conclusions Ebag9 and efp mRNA expression have changed in myocardium of rats exposed to chronic arsenic.Arsenic may has endocrine disruptor effect to female rat's myocardium.
