Diagnostic value of Sj26, Sj32 and Sj14-3-3 coding gene of Schistosoma japonicum amplified by PCR
10.3760/cma.j.issn.1000-4955.2011.04.003
- VernacularTitle:PCR扩增日本血吸虫Sj26、Sj32和Sj14-3-3抗原编码基因的诊断作用
- Author:
Shi-fei, CAI
;
Wen-gui, LI
;
Min, WANG
- Publication Type:Journal Article
- Keywords:
Schistosomiasis japonica;
Polymerase chain reaction;
Gene therapy
- From:Chinese Journal of Endemiology
2011;30(4):361-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic value of coding gene of Sj26, Sj32 and Sj14-3-3 amplified by PCR for chronic Schistosomiasis japonica. Methods The DNA was extracted from sera of 40 patients with chronic Schistosomiasis japonica, the coding gene of Sj26, Sj32 and Sj14-3-3 was amplified by PCR and identified by 1.2% agarose gel electrophoresis. DNA from the sera of 21 patients with Clonorchiasis sinensis, 13 patients with Parogonimiasis westermani and 43 healthy donors was taken as control. Results A total of 399 bp coding gene of Sj14-3-3 was amplified successfully from sera of the patients with chronic Schistosomiasis japonica,but Sj26(676 bp) and Sj32( 1270 bp) coding gene were not obtained. Control groups were all negative. Conclusions Sj14-3-3 coding gene amplified by PCR can be used for genetic diagnosis of chronic schistosomiasis.
