Preparation and clinical application of the monoclonal antibodies against the human O6 -methylguanine-DNA methyltransferase
- VernacularTitle:抗人甲基转移酶单克隆抗体的制备及临床应用研究
- Author:
Huiming, REN
;
Shouping JI
;
Subo, LI
;
Qian, WANG
;
Zepeng, LIU
;
Jun, YANG
;
Baomin, ZHANG
;
XiaoBing LI
;
YingLin, LU
;
Yangpei, ZHANG
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(2):81-84
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:To establish hybridomas secreting monoclonal antibodies(McAbs) against O6-methylguanine-DNA methyltransferase(MGMT) and to observe the relationship between MGMT expression and clinical responses to ACNU and BCNU in human brain tumors.Methods:The hybridomas were established by cell fusion.MGMT expression in 60 glioma specimens was detected by means of immunohistochemical assay.Results: Seven hybridomas secreting McAbs against MGMT were obtained.Thirty tumor specimens had no detectable or low level of MGMT expression(Mer-), while 30 specimens had high level of MGMT expression(Mer+). The Mer- patients showed more sensitive to ACNU and BCNU than the Mer+ patients.Conclusions: The high specific hybridomas secreting monoclonal antibodies(McAbs) against MGMT were established.The preliminary study indicated that MGMT negative tumors were sensitive to ACNU and BCNU, whereas MGMT positive ones were more resistant to nitrosourea drugs.
