Establishment of euglycemic clamp technique in beagle dogs
- VernacularTitle:高胰岛素-正葡萄糖钳夹技术在比格犬中的建立及应用
- Author:
Li-hou, DONG
;
Hai-feng, SONG
- Publication Type:Journal Article
- Keywords:
euglycemic clamp technique;
pharmacodynamics;
beagle dogs
- From:Bulletin of The Academy of Military Medical Sciences
2010;34(1):40-42,50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a euglycemic clamp technique in beagle dogs. Methods The euglycemic clamp technique was applied in healthy beagle dogs and the blood glucose, insulin, C-peptide, insulin and glucagon were monitored during the clamp. Results The blood glucose was controlled within the basal level.The coefficient of variation was less than 5% during the clamp. The serum insulin concentration finally reached(40.0±3.8)mIU/L stably and a significant inhibition was shown in endogenous insulin by the determination of C-peptide. But there was no significant increase in serum glucagon compared with basal values.Conclusion Methodology confirmed that the euglycemic clamp technique is successful in beagle dogs and can be applied in the study of pharmacodynamics of insulin preparations.
