Evaluation of the Broth Microdilution Method Using 2,3-Diphenyl-5-thienyl-(2)-tetrazolium Chloride for Rapidly Growing Mycobacteria Susceptibility Testing.
10.3346/jkms.2007.22.5.784
- Author:
Sun Min LEE
1
;
Jeong Man KIM
;
Joseph JEONG
;
Young Kil PARK
;
Gill Han BAI
;
Eun Yup LEE
;
Min Ki LEE
;
Chulhun L CHANG
Author Information
1. Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea. CCHL@pusan.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Mycobacteria, Atypical;
Mycobacterium Fortuitum;
Mycobacterium Abscessus;
Microbial Sensitivity Tests;
Colorimetry
- MeSH:
Anti-Bacterial Agents/pharmacology;
Cefoxitin/pharmacology;
Chemistry, Pharmaceutical/methods;
Ciprofloxacin/pharmacology;
Clarithromycin/pharmacology;
Colorimetry/methods;
Drug Resistance, Bacterial;
Korea;
*Microbial Sensitivity Tests;
Mycobacterium/*metabolism;
Mycobacterium fortuitum/*metabolism;
Tetrazolium Salts/*pharmacology
- From:Journal of Korean Medical Science
2007;22(5):784-790
- CountryRepublic of Korea
- Language:English
-
Abstract:
As the incidence of nontuberculous mycobacterial infection has been increasing recently in Korea, the importance of drug susceptibility test for clinical isolates of mycobacteria has become larger. In this study we determined the antimicrobial susceptibility patterns of clinical isolates of M. fortuitum and M. abscessus in Korea, and evaluated the efficacy of a modified broth microdilution method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC), in terms of its ability to provide accurate and easy-to-read minimal inhibitory concentration (MIC) endpoints for the susceptibility testing of rapidly growing mycobacteria. Most isolates of M. fortuitum and M. abscessus in Korea are susceptible or intermediately susceptible to amikacin, cefoxitin, ciprofloxacin, and clarithromycin. Many isolates of M. fortuitum are susceptible to doxycycline, sulfamethoxazole, and imipenem, while many M. abscessus isolates are resistant to these drugs. In the present study, the modified broth microdilution method using STC was found to be reliable, easy to read, and inexpensive for M. fortuitum and M. abscessus susceptibility testing. The modified colorimetric MIC testing method using STC was proven to be a useful surrogate for RGM antibiotic susceptibility testing.
